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anti human cd73 apc  (Miltenyi Biotec)


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    Miltenyi Biotec anti human cd73 apc
    Anti Human Cd73 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd73+apc/CD73+Antibody%2C+anti-human%2C+REAfinity/pmc13411614-164-8-25
    Average 95 stars, based on 55 article reviews
    anti human cd73 apc - by Bioz Stars, 2026-09
    95/100 stars

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    Staining:

    Article Title: Unaltered NKG2D-CAR T cell function under hypoxia in osteosarcoma in vitro
    Article Snippet: .. OS cell lines and CAR T cells were stained with combinations of fluorochrome-labeled anti-human antibodies against CD276 (B7-H3)-FITC (Clone REA1094; Miltenyi Biotec), CD73-APC (Clone AD2, Biolegend), CD274 (PD-L1)-FITC (Clone MIH2, Biolegend), HMGB1/HMG-1-PE (Clone IC1690P, R&D Systems), Galectin-9-APC (Clone 9M1-3, Biolegend), CD270 (HVEM)-APC (Clone 122, Biolegend), CD40-FITC (Clone 5C3, Biolegend), CD252 (OX40L)-PE (Clone 11C3.1, Biolegend), CD137L (4-1BB Ligand)-APC (Clone 5F4, Biolegend), MICA-APC (Clone 159,227, R&D Systems), MICB- APC (Clone 236,511, R&D Systems), UBLP-1- Alexa Fluor 488 (Clone 170,818, R&D Systems), ULBP-2/5/6-PE (Clone 165,903, R&D Systems), ULBP-3-PE (Clone 166,510, R&D Systems), ULBP-4-APC (Clone 709,116, R&D Systems), NKG2D-PEVio770 (Clone REA797, Miltenyi Biotec), CD25-PEVio770 (Clone REA945, Miltenyi Biotec), PD1-PEVio770 (Clone REA1165, Miltenyi Biotec), NTBA-PE (Clone NT7, Biolegend), TIM-3-FITC (Clone F38-2E2, Biolegend), CD107a-PE (Clone H4A3, Biolegend), CD69-PECy7 (Clone FN50, Biolegend). .. Intracellular staining was performed using GranzB-Pacific Blue (Clone GB11, Biolegend) and Fixation and Permeabilization Buffer Kit I (R&D system).

    Article Title: Unaltered NKG2D-CAR T cell function under hypoxia in osteosarcoma in vitro.
    Article Snippet: .. OS cell lines and CAR T cells were stained with combinations of fluorochrome-labeled anti-human antibodies against CD276 (B7-H3)-FITC (Clone REA1094; Miltenyi Biotec), CD73-APC (Clone AD2, Biolegend), CD274 (PD-L1)FITC (Clone MIH2, Biolegend), HMGB1/HMG-1-PE (Clone IC1690P, R&D Systems), Galectin-9-APC (Clone 9M1-3, Biolegend), CD270 (HVEM)-APC (Clone 122, Biolegend), CD40-FITC (Clone 5C3, Biolegend), CD252 (OX40L)-PE (Clone 11C3.1, Biolegend), CD137L (4-1BB Ligand)-APC (Clone 5F4, Biolegend), MICA-APC (Clone 159,227, R&D Systems), MICB- APC (Clone 236,511, R&D Systems), UBLP-1- Alexa Fluor 488 (Clone 170,818, R&D Systems), ULBP-2/5/6-PE (Clone 165,903, R&D Systems), ULBP-3-PE (Clone 166,510, R&D Systems), ULBP4-APC (Clone 709,116, R&D Systems), NKG2D-PEVio770 (Clone REA797, Miltenyi Biotec), CD25-PEVio770 (Clone REA945, Miltenyi Biotec), PD1-PEVio770 (Clone REA1165, Miltenyi Biotec), NTBA-PE (Clone NT7, Biolegend), TIM-3-FITC (Clone F38-2E2, Biolegend), CD107aPE (Clone H4A3, Biolegend), CD69-PECy7 (Clone FN50, Biolegend). .. Intracellular staining was performed using GranzB-Pacific Blue (Clone GB11, Biolegend) and Fixation and Permeabilization Buffer Kit I (R&D system).

    other:

    Article Title: Mesenchymal cells support the early retention of primary alveolar type 2 cells on acellular mouse lung scaffolds
    Article Snippet: CD73 (APC) , Rat (REA778) , 1:50 , Miltenyi Biotech (130-111-518).

    Bioprocessing:

    Article Title: Co-culture of multiple myeloma cell lines and bone marrow mesenchymal stem cells in a 3D microgel environment.
    Article Snippet: This study reproduces the complex relationships between tumour plasma cells and their bone marrow microenvironment in multiple myeloma in vitro.. These relationships are established both with other cells and with the extracellular matrix and are key factors in tumour progression, generating resistance to antitumour drugs in the cellular and non-cellular environments.. This paper proposes a 3D microenvironment model designed to capture the main components of the multiple myeloma tumour microenvironment.



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    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers <t>(CD73,</t> CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
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    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers <t>(CD73,</t> CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
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    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers <t>(CD73,</t> CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
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    Image Search Results


    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Journal: bioRxiv

    Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells

    doi: 10.64898/2026.06.25.733625

    Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

    Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay